One of the major ailments of very common occurrence amongst the people of Northern Pakistan is the diseases of urinary tract including kidney with the formation of calculi or stones in majority of the cases also known as urolithiasis. It is caused by missense mutations in Adenine phosphoribosyltransferase (APRT) enzyme, encoded by APRT gene. In this project, Ethnobotanical data was collected through informed consent semi-structured interviews. Nine plant species viz: Bryophyllum calycinum, Centella asiatica, Tribulus terristris, Berberislycium, Solanum nigrum, Phylanthus nirruri, Trianthema portulacastrum, Aerva lanata and Achyranthes aspera were identified to cure urolithiasis. The effective anti-urolitiatic chemical constituents of plants were downloaded and docked with APRT. Interactions of chemical constituents with APRT were analyzed. In each docked complex the common interacting amino acid residues were Threonine25, Proline85, Proline77, Leucine28, Leucine62, Isoleucine24, Leucine84, Histadine105, Alanine86 and phenylalanine75. Results showed that each chemical constituent best fit in the pocket of enzyme, interacted with each amino acid residue of mutated APRT enzyme’s pocket and does not leave the complex that demonstrates its stability and soundness. On the basis of docking results it is suggested that all these chemical constituents of plant can be used to treat Urolithiasis.
 

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