Treatment of infertility-related hormonal dysfunction in men requires an understanding of the hormonal basis of spermatogenesis. The best method for accurately determining male androgenization status remains elusive. This study is designed to study levels of follicle-stimulating hormone (FSH), Luteinizing hormone (LH), Dehydroepiandrosterone (DHEA) and Testosterone (T) in seminal plasma and microscopic examination of seminal fluid have been measured, in different groups of infertile patients. Semen samples were collected from the infertile and fertile subjects. The samples will be collected from (44) subjects (34) as infertile patients (12 Asthenozoospermic, 10 Oligozoospermic, and 12 Azoospermic) and 10 Normozoospermic subjects as fertile (control group). Then the hormones levels were measured by using Enzyme Linked Immunosorbent Assay (ELISA). The results of hormones levels in seminal plasma showed that there was a highly significant increase (p<0.001) in the levels of FSH and LH by comparison between fertile and infertile men subgroups. Hormones levels showed that there was a significant increase (p<0.05) in the levels of DHEA and Testosterone in comparison between fertile men and infertile men subgroups. The Significant correlations between the reproductive hormones (FSH, LH, DHEA, Testosterone) in seminal plasma and main seminal fluid parameters were found with sperm concentration, sperm motility and morphologically normal sperm in fertile and in the infertile group as a whole. From the results obtained, it can be concluded that: the determination of hormones (FSH, LH, DHEA, and Testosterone) of infertile men are of great importance to determine the degree of infertility.
 

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