Tuberculosis is an infectious disease that influences humanity for more than 4,000 years. It is a prolonged disease affected by the bacillus Mycobacterium tuberculosis and extends from person to person through air. Pitiable knowledge between health care workers as well as insufficient HICPAC might be basic to improve the hazard of hospital acquired Tuberculosis spread. A descriptive cross sectional study design was used to assess the Knowledge attitude and practice of Health care workers. Data was collected through a questionnaire. The overall 150 health care workers were evaluated for awareness, assertiveness as well as observation on Tuberculosis contamination. Knowledge on Tuberculosis contamination mechanism between practically 77.3 %( 1.23±.420) of Healthcare workers recorded “worthy” amounts of awareness while 22.7%of them had “deprived” awareness. The awareness was considerably related by instructive grade, also Tuberculosis teaching and coordination acknowledged. Most of the health care workers 61.3 %( 1.39±.489) may be confident in Tuberculosis contamination controllers. 38.7% of Healthcare workers were diseased with Tuberculosis. Usage of breathing apparatus between the Healthcare workers were restricted as well as triage of Tuberculosis respondents were absent. Total practice and awareness of health care workers towards tuberculosis contamination controllers wasn’t suitable by the awareness actually not as good as between non-therapeutic also junior workers. Consistent orientation and ability constructed teaching towards contamination controllers for entirely units of health care workers might increase contamination control performs in the well-being services In addition, TB contamination control guideline and policy is mentioned to confirm real infection control measures in wellbeing services.