Promising pharmacological treatment of disk degeneration depends on development of appropriate materials and obtaining viable and proliferative nucleus pulposus/annulus fibrosus tissues with preservation of extracellular matrix compounds. This study aims to explain how drug and/or similar material impregnated polymeric scaffolds can be tested using primary cell cultures derived from human nucleus pulposus and annulus fibrosus tissues at pharmacomolecular level. For this purpose; we first explained how to prepare human primary cell culture setups. We remarked the physicochemical parameters for testing drugs and scaffolds containing drug-like substances. How to conduct the molecular analyzes of samples of cell cultures was explained. In this study; we explained very basically how to make in vitro analyzes for the development of the ideal drugs and/or polymeric biomaterials that can be used in clinical practice for the purpose of preventing disc degeneration. Moreover, medical pharmacologists, orthopedic and brain surgeons taking part in such studies can set up and analyze their own studies.
 

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