Blood transfusion centers are vital part of the health system and donors make a unique involvement in saving lives and enhancing affected persons health. The study is to ensure a safe and adequate blood supply. Blood transfusion services must be established to maintain a safe pool of free and voluntary blood donors. A cross-sectional design was used in this study. The research was conducted at a private university in Lahore. The target population of this research was students. 385 students from different departments participated in this study. Data was analyzed on SPSS version 21. The results showed that only 24.4% of students have inadequate knowledge about blood donation. The attitude of the participant was not too good towards blood donation. 64.6% of participants show a positive attitude. The majority of university students have inadequate knowledge about blood donation. Their attitude was positive toward blood donation. Most students show their willingness for blood donation. Their Practices were average but if we compare it with past study, then it shows a valuable change.