The aim of study was to investigate the effect of thiamine (Vitamin B1) on antioxidative activities of neutrophils and the hematological parameters following the lead toxicity in rats. Forty Wistar rats were randomly assigned into 4 groups (n=10). Group A served as control, Group B received 30 mg/l thiamine, Group C received 8.5 mg/l lead acetate, group D treated by thiamine along with lead acetate by oral gavages for 20 consecutive days. To evaluate the impact of oxidative stress and lipid peroxidation, the levels of thiobarbituric acid reactive substances (TBARS) were measured in serum samples that were collected at time intervals after 0, 10 and 20 days. Compared with basal level on day 0, lead administration (Group C) resulted in a significant increase of TBARS in blood (P<0.05). On 10th and 20th days in group C and 20th day in group D, a significant increase were also observed in TBARS value than control group (P<0.05). PCV and RBC values did not show significant changes. Also, there were significant increases of neutrophil counts and significant decreases of the lymphocyte counts on days 10 and 20 in group C, in comparison with day 0 and controls (P<0.05). The results of the present study in group D indicate thiamine’s ability to appease lead-induced alterations in blood tissue. This study demonstrates that exposure with lead results in changes in blood cells. Thiamine supplementation can induces ameliorative effects during this susceptible period.
 

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