Toxoplasma gondii; is one of the most prevalent abortificiant human protozoan inducing complicated pregnancy, has usual colonial population of three virulent types; I, II, and III; with dissimilar ecological and zoonotic modes of transmission. The objective of the present study is directed towards the biological typescript of T. gondii local Egyptian isolates which collected from complicated pregnant women by using mice passage and kittens. The current study deals with isolation and biological characterization of T. gondii isolates from complicated pregnant Egyptian women. Placenta land or fetal tissue samples were collected from one hundred and six women attending the labour ward of (Beni-Suef General Hospital and Kasr Al-Aini Hospital-Egypt), which were suffering from complicated pregnancy. The study includes G1, G2, G3 and G4 which symbolized the abortion corresponding to the 1st, 2nd and 3rd trimesters with congenital anomalies. The fresh placental tissue samples were microscopically examined after pepsin digestion; the confirmed bradyzoites containing samples were bio-assayed by intraperitoneal (IP) passage in mice as intermediate host model to conclude the mice Lethal Dose [LD] and the tissue cyst forming characters. Wherever, the isolates successive lypass in mice were bio-assayed by oral inoculation in a toxoplasma free un-weaned kittens for confirmation the oocysts shedding properties. Accordingly, different isolates were biologically typescript to one of type I, II or III. The results showed that seven local isolates (6.6%) separated from complicated pregnant cases and were biologically characterized in mice and kittens as cystogenictype-II, with ecological conclusion of transmission of infection mainly through insufficient cooked meat harboring viable tissue cyst.
 

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