The aim of the current study was to examine the effect of whey protein supplement on anthropometric measurements as well as on resting metabolic rate. Forty-one Saudi female students between 18-24 years old recruited for this case controlled study. Twenty-eight students were given 24 grams of whey protein supplement daily for 21 days. These subjects were divided into three groups based on their body mass index. Group one with normal weight, group two with overweight, group three with obesity. The remaining subjects who had normal weights served as controls. Anthropometric measurements, blood analysis, resting metabolic rate were measured in all subjects at baseline and at the end of the study. Twenty-four hours recall was conducted four-five times during the study to determine macronutrients intake for all subjects. Fat body mass decreased significantly (P= < 0.024) in group one. Resting metabolic rate increased significantly in group one and three subjects (P= < 0.001). Group two and three were not regularly eating breakfast by 42.9% and 50%, respectively. Dietary whey protein supplementation even during a three-week intervention showed significant increase in resting metabolic rate among females’ subjects with normal weight as well as subjects with obesity.